DNA+Structure


 * Polymerase Chain ReactionPolymerase Chain ReactionDNA structure and technology**

wil

1/30 ||= Conduct Enzyzme IA ||  ||   || 2/1 ||= Conduct Enzyzme IA ||  ||   || 2/5 ||= Conduct Enzyzme IA ||  ||   || 2/7 || Enzyme IA and Complete Enzyme Study Guide ||  ||   || 2/11 ||= Begin DNA Structure || - Begin by examine the following link: DNA Structure Animation -Use the following document to work on pages 23-32 of the Study Guide. ||  || 2/13 || DNA Structure and work on IA ||   ||   || 2/20 || DNA Structure Exam DNA Models || Build DNA with K'nex DNA Replication and Transcription set ||  || 2/22 || DNA Extraction Lab || ||   || 2/26 || Intro to DNA Replication || - review DNA Structure - Error Bars for Graphs - DNA Replication Process (McGraw-Hill) ||  || 2/28 || DNA Replication || - DNA Structure Quiz - ||   || 3/11 || DNA Fingerprinting Activity || DNA Fingerprinting Video || bring personal headphones or earbuds to future classes for private viewing of content videos. || 3/13 || DNA Transcription || DNA Khan Lecture || IA DUE AT END OF WEEK   (2 hard copies only)  || 3/15 || DNA Transcription and Translation || View the following videos How Transcription Works How Translation Works || Work on Transcription and Translation section of study guide || 3/19 || Review DNA Begin Respiration || Go to Cellular Respiration for materials on next unit ||  || 3/21 || DNA Exam ||  ||   ||
 * **Date** ||= **Daily Topic** || **Materials** || **Homework** ||
 * 1/29
 * 1/31
 * 2/4
 * 2/6
 * 2/8
 * 2/12
 * 2/19
 * 2/21
 * 2/25
 * 2/27
 * 3/1
 * 3/12
 * 3/14
 * 3/18
 * 3/20

DNA Replication Process

DNA Replication Animation I I

__**Assessment statements**__

DNA Structure 3.3.1 Outline DNA nucleotide structure in terms of sugar (deoxyribose), base and phosphate. 3.3.2 State the names of the four bases in DNA. 3.3.3 Outline how DNA nucleotides are linked together by covalent bonds into a single strand. 3.3.4 Explain how a DNA double helix is formed using complementary base pairing and hydrogen bonds. 3.3.5 Draw and label a simple diagram of the molecular structure of DNA. 4.1.1 State that eukaryote chromosomes are made of DNA and proteins. 7.1.1 Describe the structure of DNA, including the antiparallel strands, 3’–5’ linkages and hydrogen bonding between purines and pyrimidines. 7.1.2 Outline the structure of nucleosomes. 7.1.3 State that nucleosomes help to supercoil chromosomes and help to regulate transcription. 7.1.4 Distinguish between unique or single-copy genes and highly repetitive sequences in nuclear DNA.

DNA technology 4.4.1 Outline the use of polymerase chain reaction (PCR) to copy and amplify minute quantities of DNA. 4.4.2 State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. 4.4.3 State that gel electrophoresis of DNA is used in DNA profiling. 4.4.4 Describe the application of DNA profiling to determine paternity and also in forensic investigations. 4.4.5 Analyse DNA profiles to draw conclusions about paternity or forensic investigations. 4.4.6 Outline three outcomes of the sequencing of the complete human genome.

Use the following links to complete pages 33-39 of your Unit Study Guide 3.4 DNA Replication 7.2 DNA Replication DNA Replication

Base Pairing

Chargaff Rule How DNA is Packaged

Processing Gene Information Animation

Protein Synthesis Animation

Spicing RNA Animation

How Transcription Works

How Translation Works

Protein Synthesis

Transcription 3-D

translation 3-D

DNA Replication

Polymerase Chain Reaction

DNA Structure Animation Khan Academy Video - DNA Chargaff's Ration (video) Coding Sequences in DNA (video) DNA Paired Strands (video) Watson on Pairing Base Pairs (video) DNA Triplet Code (video)

__**What You Need to Know**__ ** DNA Structure ** Draw the basic structure of a DNA nucleotide. Draw the molecular structure of deoxyribose and number the carbon atoms. Identify the four bases of DNA based on the numbers of rings (purines vs. pyrimidines) and the number of hydrogen bonds it can form. Outline how the DNA nucleotides are linked together by covalent bonds into a single strand. Know which bases are capable of complimentary pairing. How many bases are there per complete turn of the helix? Outline the structure of nucleosomes, referring to the correct number of histone proteins. Describe what is meant by “anti-parallel.” Identify the 5’ and 3’ ends of a DNA molecule. Describe the structure of the DNA found inside mitochondria and chloroplasts, and explain how this is evidence for the endosymbiotic theory. ** DNA technology ** What are restriction enzymes? Where are they found naturally? What is their role in nature? What is a restriction site? Define palindrome. How are restriction enzymes named? Contrast sticky vs. blunt ends. Why are restriction enzymes used in DNA electrophoresis? What is electrophoresis? For what purposes is it used? Based on what properties are fragments separated? What are the functions of the buffer and loading dye? Describe the process of DNA fingerprinting. Read a DNA fingerprint to determine relatedness or forensic guilt. Be able to read a pipette to determine its range of volume measurement.

7.1: DNA Structure

7.2: DNA Replication

[|7.3 & 7.4 Transcription & Translation]

DNA Structure (core and AHL) by Stephen Taylor

__**Unit Activities List:**__ [|Nucleic acids web assignment] (doc)   [|DNA extraction lab] (pdf)    [|Race for the Double Helix video] (doc)   [|DNA history readings questions] (doc) DNA history [|article 1], [|article 2] and [|article 3] (all pdf). [|DNA structure notes template] (doc)  [|DNA structure notes presentation]   [|DNA components worksheet] (doc)   [|DNA models] (doc)   DNA diagram labeling and quiz   [|Play Doh models] (doc)   [|Human genome project web assignment] (doc)   [|HGP and Gene Identification] (doc)   [|Mystery genes] (doc)   [|Electropherograms for mystery genes] (pdf)   [|Modeling electrophoresis lab] (doc)   [|Electrophoresis notes] (doc). Here's the [|first link], the [|reading for the second part] (pdf), and the [|presentation for the third part] (ppt). [|Restriction enzyme notes and problems] (doc) [|DNA sequences for practice problems] (doc) [|Restriction enzyme site finder challenge] (doc) [|Sequences for the restriction enzyme site finder challenge] (doc) [|Answer key to the site finder challenge] (doc) [|Pipette practice lab and skills worksheet] (doc)  [|DNA fingerprinting lab] (doc)   [|Idealized results for the DNA fingerprinting lab] (doc)

__**Key Terms:**__
 * 3' || micropipette ||
 * 5' || mitochondria ||
 * adenine || naked DNA ||
 * agasose gel || nitrogenous base ||
 * antiparrallel || nucleic acid ||
 * chloroplast || nucleoid ||
 * chromatin || nucleosome ||
 * chromosome || nucleotide ||
 * covalent bond || nucleus ||
 * crick || palandrome ||
 * cytosine || pentose ||
 * deoxyribonucleic acid || phosphate ||
 * deoxyribose || plasmid ||
 * DNA fingerprint || purine ||
 * electrophoresis || pyrimidine ||
 * Franklin || restriction enzymes ||
 * guanine || restriction site ||
 * helicase || thymine ||
 * histone || tracking dye ||
 * hydrogen bond || Watson ||
 * microliter || X ray diffraction ||

__**Helpful Links:**__ ** DNA Structure ** [|Journey Into DNA] [|DNA anatomy] [|DNA Discovery Modules] [|DNA Coiling To Form Chromosomes] [|Chromosome Description]

** DNA technology ** [|The Human Genome Project animations] [|Cycle sequencing] [|DNA restriction] [|Gel electrophoresis] [|PCR Mutagenesis] [|PCR] [|PCR] [|Sanger sequencing] [|Paternity Testing] [|Plasmids, Restriction Digestion, DNA gels] [|Gene Technology] [|BLAST search] [|Scientist for Better PCR] [|Sequence For Yourself] [|Gel Electrophoresis] [|DNA Sequencing]